ABSTRACT
Priestia megaterium MWU16-30321 was isolated from a mixed bacterial culture in a cranberry stem gall in Massachusetts following a severe winter. The genome is 5,623,390 bp in size and putatively encodes indole-3-acetic acid acetyltransferase, a key enzyme in tryptophan-dependent indole-3-acetic acid production.
ABSTRACT
Pseudomonas sp. strains MWU12-2319 and MWU12-2311 were isolated from the soil of a wild cranberry bog in the Cape Cod National Seashore as part of a culture-dependent bacterial population survey. The genomes exceed 7 Mbp and contain putative gene clusters for the biosurfactant orfamides A and C.
ABSTRACT
Pseudomonas sp. strains MWU12-2029, MWU12-3088, and MWU12-3091 were isolated from wild and cultivated cranberry bog soils in southeastern Massachusetts. The three isolates are closely related to Pseudomonas kribbensis, a not validly published member of the P. fluorescens group, and contain three putative insecticidal protein genes, including the toxin complex A gene (tcaC).
ABSTRACT
Pseudomonas sp. strain MWU13-3659 was isolated from cultivated cranberry bog soil in Massachusetts, USA. Its closest known relative is Pseudomonas entomophila (digital DNA-DNA hybridization [d4 formula] value of 57.2% and average nucleotide identity based on BLAST value of 93.90), and its genome contains putative gene clusters for the production of polyketides, siderophores, and cyclic lipopeptides that have insecticidal activity in other proteobacteria.
ABSTRACT
Here, we present the draft genome sequences of Pseudomonas sp. strains MWU12-2020 and MWU12-3103b, isolated from the rhizospheres of wild and cultivated cranberry bogs in southeastern Massachusetts; these strains are unrelated to known Pseudomonas species. The genomes of both isolates exceed 6 Mbp and contain predicted ice nucleation and type VI and III secretion system genes.
ABSTRACT
Chromobacterium sp. strain IRSSSOUMB001 with potent insecticidal activity was isolated from Anopheles gambiae s.l. in Burkina Faso. The draft genome is 5,090,822 bp and encodes predicted genes for hydrogen cyanide production, haemolysin, a T3SS, and yopE, which are potential virulence factors against mosquitoes.
ABSTRACT
Curtobacterium sp. strain MWU13-2055 was isolated from cranberry fruit surfaces in the Cape Cod National Seashore. The genome is 4 Mbp long with a large number of genes predicted to be devoted to heavy metal resistance, including the copAZ operon and translocases for Pb, Cd, Zn, Hg, and Cu.
ABSTRACT
Pseudomonas moorei has been used to detoxify recalcitrant environmental contaminants from the pharmaceutical industry. Two P. moorei strains were isolated from soil in the pristine wild cranberry bogs of the Cape Cod National Seashore that putatively encode genes for degradation of 4- and 5-chlorosalicylates, acetaminophen, and diclofenac.
ABSTRACT
Pseudomonas sp. strains MWU13-2100 and MWU13-2105 were isolated from a wild cranberry bog with Pipestone loamy coarse sand soil in Truro, Massachusetts, and taxonomically assigned based on whole-genome sequences. The draft genomes are most closely related to P. batumici (41.4% and 41.8% dDDHd4), but with only 50.8 dDDHd4 to each other.
ABSTRACT
Pseudomonas spp. are dominant in many soils, but little is known about how they interact with other members of the soil microbiome. Pseudomonas sp. strain MWU13-2516, isolated from a wild cranberry bog in Massachusetts, has predicted genes for hemolysins, usually associated with pathogens, and type 6 secretion systems.
ABSTRACT
Pseudomonas sp. strain MWU15-20650 was isolated from wild cranberry fruit surfaces in the Cape Cod National Seashore. The draft genome is 6.2 Mbp, with a G+C content of 59%, and contains predicted genes for type VI secretion systems and an N-acyl-homoserine lactone acylase. The closest known relative is Pseudomonas haemolytica.
ABSTRACT
Pseudomonas protegens strain MWU12-2233 was isolated from wild cranberry fruit surfaces in Provincetown, MA. The genome contains putative hydrogen cyanide synthase and type VI secretion systems which can act symbiotically on plant health by suppressing competitors, indicating a role in indigenous microfloral disease and insect pest suppression.
ABSTRACT
Pseudomonas sp. strains MWU12-2037 and MWU12-2345 were isolated from peat and sandy bog soils in wild cranberry bogs in the Cape Cod National Seashore (Massachusetts, USA) as part of a culture-dependent survey of relatively unexplored wetlands soil microbiomes. Both genomes exceeded 7 Mbp.
ABSTRACT
Two non-pigmented strains in the genus Chromobacterium, MWU14-2602T and MWU13-2610T, were isolated from wild cranberry bogs in the Cape Cod National Seashore, USA. The isolates were characterized by genomic and phenotypic analyses, the results of which indicated that they represent two novel species. Based on total genome sequences, the closest relatives were in the Chromobacterium amazonense group, which includes the recently described Chromobacterium paludis. Whole genome sequences were compared by genome blast distance phylogeny, digital DNA-DNA hybridization and average nucleotide identity analyses with each other and with the type strains of their nearest species. MWU14-2602T and MWU13-2610T fell well below the accepted cutoff values for species relatedness, clearly indicating that they represent novel species. Although little is known about these organisms in situ, under laboratory conditions, MWU13-2610T produced a modest amount of HCN and was strongly positive for exoprotease activity, whereas MWU14-2602T did not produce HCN or exoproteases. The predominant fatty acids for both isolates were summed C16â:â1ω7cis/C16â:â1ω6cis. Both isolates produced siderophores and pyomelanin pigment on rich media, and neither was haemolytic on sheep blood agar. We propose the names Chromobacterium alticapitis sp. nov. (type strain MWU14-2602T=ATCC TSD 260T=CCOS 1979T) and Chromobacterium sinusclupearum sp. nov. (type strain MWU13-2610T=ATCC TSD-259T=CCOS 1981T) for these taxa.
Subject(s)
Chromobacterium , Vaccinium macrocarpon , Animals , Bacterial Typing Techniques , Base Composition , DNA, Bacterial/genetics , Fatty Acids/chemistry , Nucleic Acid Hybridization , Phylogeny , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Sheep , Vaccinium macrocarpon/genetics , WetlandsABSTRACT
Four Pseudomonas spp. and Xanthomonas arboricola were isolated from cranberry stem galls in Carver, MA, and taxonomically assigned at the genus level based on the 16S rRNA sequence and phenotypes. X. arboricola had not been associated previously with cranberry stem galls or any cranberry disease.
ABSTRACT
The surfaces of plants are colonized by a rich diversity of microbes but are largely unexplored. Here, we present the draft genome sequences of five Pseudomonas spp. isolated from cultivated cranberry fruit surfaces. Although the isolates represent four different species, their genomes all contain conserved iron sequestration and uptake genes.
ABSTRACT
The precipitous drop in the cost of genomic sequencing and the concomitant availability of computational methods for comparing genome-level data has made the accurate taxonomic placement of bacteria affordable and relatively rapid. Inaccurate taxonomic placement of bacteria has serious implications in clinical, environmental, and regulatory microbiology, but it can also adversely affect interpretation of research results. The quorum biosensor strain CV026 was derived from an isolate of Chromobacterium that was labeled as C. violaceum ATCC 31532, and is catalogued by the ATCC under that species name. Nearly 200 papers have been published that use CV026 as an indicator for quorum sensing activity in many Gram negative bacteria, but the inability of C. violaceum strains to complement the quorum sensing mutation in CV026 has called the taxonomic placement of the parent strain into question. We used molecular phylogeny and a large number of metabolic and phenotypic characters to demonstrate that Chromobacterium strain ATCC 31532 is a member of species Chromobacterium subtsugae.
ABSTRACT
The study of chemical bioactivity in the rhizosphere has recently broadened to include microbial metabolites, and their roles in niche construction and competition via growth promotion, growth inhibition, and toxicity. Several prior studies have identified bacteria that produce volatile organic compounds (VOCs) with antifungal activities, indicating their potential use as biocontrol organisms to suppress phytopathogenic fungi and reduce agricultural losses. We sought to expand the roster of soil bacteria with known antifungal VOCs by testing bacterial isolates from wild and cultivated cranberry bog soils for VOCs that inhibit the growth of four common fungal and oomycete plant pathogens, and Trichoderma sp. Twenty one of the screened isolates inhibited the growth of at least one fungus by the production of VOCs, and isolates of Chromobacterium vaccinii had broad antifungal VOC activity, with growth inhibition over 90% for some fungi. Fungi exposed to C. vaccinii VOCs had extensive morphological abnormalities such as swollen hyphal cells, vacuolar depositions, and cell wall alterations. Quorum-insensitive cviR - mutants of C. vaccinii were significantly less fungistatic, indicating a role for quorum regulation in the production of antifungal VOCs. We collected and characterized VOCs from co-cultivation assays of Phoma sp. exposed to wild-type C. vaccinii MWU328, and its cviR - mutant using stir bar sorptive extraction and comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (SBSE-GC × GC-TOFMS). We detected 53 VOCs that differ significantly in abundance between microbial cultures and media controls, including four candidate quorum-regulated fungistatic VOCs produced by C. vaccinii. Importantly, the metabolomes of the bacterial-fungal co-cultures were not the sum of the monoculture VOCs, an emergent property of their VOC-mediated interactions. These data suggest semiochemical feedback loops between microbes that have co-evolved for sensing and responding to exogenous VOCs.
ABSTRACT
Exploration of novel environments such as low-pH wild cranberry bog soils yields a rich diversity of bacteria, including Pseudomonas spp. Here, we present the draft genome sequence of Pseudomonas sp. strain MWU12-2323, isolated from wild cranberry plant rhizosphere. The genome has secondary metabolite genes encoding carbohydrate polymer-degrading enzymes.
